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Fig. 4. The Fkbp8-/- neural tube is ventralized. Sections of
E11.5 wild type and Fkbp8-/- neural tubes were analyzed by
in situ hybridization. Sections at caudal levels of the neural tube just
anterior to the hindlimbs are shown. Markers of dorsal fates, Wnt1
(A,B) and Gdf7 (data not shown) are not expressed in
Fkbp8-/- mutants. In the mutant, dorsal cells adopt
lateral fates marked by Pax6 and Dbx1 (D,F), and lateral
cells acquire ventral fates marked by Foxa2 (H) and Nkx2.2
(data not shown). Expression of MSX1 and MSX2 in the E10.5 dorsal neural tube,
monitored by immunofluorescence with an antibody that recognizes both
proteins, is relatively unaffected in Fkbp8-/- mutants
despite changes in neural tube morphology (I,J). Scale bars: 65 µm.
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