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Fig. 7. Gene specific RT-PCR analyses of VEGFA receptors in epiphyseal
chondrocytes. Total RNA was extracted from epiphyseal chondrocytes. RT-PCR
reactions were performed in the presence of reverse transcriptase (RT) (+) or
in its absence () (as a control for genomic DNA contamination).
Cd31 was also amplified as a control for endothelial cell
contamination. Nucleotide size markers are indicated at left. Amplified bands
are only seen in the Vegfr3, Nrp1 and Nrp2 lanes, as
indicated by asterisks.