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Fig. 1. Analysis of Parkin amino acid sequence conservation and developmental
expression. (A) Schematic of human and Drosophila Parkin and PARK2
proteins. The ubiquitin-homology domain is an N-terminal domain, followed by
the first RING finger domain (R1), an in between ring (IBR) domain, and a
second RING finger domain (R2). Similarity and identity values for each domain
are shown. (B) Multiple sequence alignment of human, mouse and
Drosophila Parkin proteins shows a high degree of conservation
throughout the lengths of the proteins. Solid boxes indicate identities;
shaded boxes represent similarities. (C) Developmental northern analysis of
parkin expression. A 1.6 kb parkin transcript is first
detected in 0-2 hour embryos but is undetected in subsequent embryonic stages.
parkin is again expressed during third instar larval (3L), early (E),
mid (M) and late (L) pupal stages, as well as in adults. RP49 was
used as a loading control on the same filter.
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