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Fig. 3. NCCs develop in BMPRIA NCC mutant embryos. (A,C,E) Wild-type, stage-matched control embryos for Wnt1-Cre; Bmpr1aflox/null mutants (B,D,F). (A,B) Expression of Crabp1 and other neural crest markers showed normal NCC specification in mutants. (C,D) Immunostaining for neurofilaments with the 2H3 antibody showed proper differentiation of NCCs in mutants. (E,F) Mutant embryos expressing the R26R reporter, to label NCCs, show a normal distribution of NCCs and proper formation of many target tissues, including pharyngeal arches (arrows) and dorsal root ganglia (arrowheads).





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