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Fig. 8. Epicardium appears normal in BMPRIA mutants. (A,B) NCCs appear in the same limited quantities in mutants as in control embryos at E9.5 (A) and E11.5 (B: compare to Fig. 7A,B). (C-F) Histological analysis (transverse sections) of the epicardium (e) at E11.5 shows no significant difference in the appearance of the epicardium of mutant tissue (D,F) which shows a clear ventricular myocardial phenotype (D, ivs: interventricular septum, pp: parietal pericardium). Dotted lines in E,F delineate the boundary between epicardium and compact ventricular myocardium. (G,H) In situ hybridization for epicardin also shows no defect in mutant epicardium (insets show that hybridization is specific to epicardial layer, pericardium was removed prior to in situ hybridization). (I,J) Immunohistochemistry for the WT-1 protein shows no decrease in expression in the mutant in either the epicardium or the coelomic epithelial cells of the parietal pericardium (cm, compact ventricular myocardium). PA1, first pharyngeal arch; a, atrium; e, epicardium. All paired images are shown at identical magnification.





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