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Fig. 2. DN-xBrg1 and xBrg1MO injections have similar effects on embryonic morphology. (A-C) Morphology of tadpoles (stage 37/38) injected with DN-xBrg1, xBrg1MO or standard MO. Embryos were injected in both blastomeres at the two-cell stage and raised to tadpoles. (A) Bottom three embryos were injected with DN-xBrg1, while the top embryo was uninjected. (B) xBrg1MO (20 ng) injected tadpoles. xBrg1MO-injected tadpoles display similar defects to DN-xBrg1, while standard MO (C) injected ones do not show apparent defects. (D) Reduction of endogenous xBrg1 protein by xBrg1MO. Embryos were injected with 25 ng of xBrg1 or standard MO in both blastomeres at stage 2 and harvested at the indicated stages. Ten embryos were used per sample, with one embryo-equivalent of lysate loaded per lane for western blotting.





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