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Fig. 3. Embryos depleted of xBrg1 fail to produce primary neurons. Embryos were injected with DN-xBrg1 (A-C), xBrg1MO (D-F) or standard MO (G-I) in one blastomere at stage 2. ß-galactosidase mRNA was co-injected and X-Gal staining (blue) was performed to reveal distribution of injected materials. Embryos were analyzed for expression of Sox2 (A,B,D,E,G,H,J) and N-tubulin (C,F,I,K; stage 15). Embryos in J and K were injected with xBrg1MO and xBrg1 mRNA. Dorsal views with injected side facing rightwards.





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