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Fig. 4. Loss of Brg1 increases cell proliferation. (A-D) Whole-mount stage 14 embryos immunostained (brown) to detect phosphorylated histone H3 (PH3) after injection of standard MO (A) or xBrg1MO (B-D). (C,D) Lateral views of an xBrg1MO-injected embryo: (C) uninjected side; (D) xBrg1MO-injected side. (E) Transverse section of embryo in B, showing the injected side facing rightwards. Black arrowheads mark PH3-positive cells. n, notochord; s, somite. (F-H) Cell division was blocked by adding hydroxyurea (HU) from stage 12.5 until fixation at stage 15. (F) PH3-immunostained embryo showing cell cycle is efficiently blocked by HU treatment (xBrg1MO+ß-galactosidase injection, blue; PH3 stain, brown). (G,H) xBrg1MO-injected embryos were raised in the presence of 30 mM HU from stage 12.5 and analyzed for Sox2 (G) and N-tubulin (H) expression. (I,J) Whole-mount TUNEL staining (brown) after injection of standard MO or xBrg1MO. A,B,F-J are dorsal views with injected side facing rightwards.





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