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Fig. 6. The requirement of Brg1 for neurogenesis is conserved in mammalian P19 cells. P19 cells were transfected with plasmids encoding mouse NeuroD2 (A-C), mouse NeuroD2 and DN-hBrg1 (D-F), together with mE12 and GFP expression vectors. Three days after transfection, cells were analyzed by TuJ1 immunostaining (A,D,G) and GFP expression (B,E,H). C, F and I are overlays of images A and B, D and E, and G and H, respectively. (J) TuJ1-positive cells were scored as a percentage of GFP-positive transfected cells. A total of between 750 and 2200 GFP-positive cells were counted for each type of transfection. P19 cells transfected with GFP vector alone (G-I) or with GFP and E12 vectors (but not with NeuroD2) yielded less than 1 TuJ-positive cell per 1.8x105 cells assayed in each experiment. Data shown in J are the average of three experiments.





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