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Fig. 2. Growth and patterning phenotypes of Hhip1^-/- and Hhip1^-/-;Ptch1^+/- neural tube at E10.5. (A) Summary of the expression patterns of the markers used in this study to identify progenitor domains and neuronal precursor populations (^*Lim1/2 has other expression domains more ventrally). (B,C) Cross-sections of neural tube from wild-type (B) and Hhip1^-/-;Ptch1^+/- (C) embryos. Green, Pax7 protein; blue, nuclei stained with Topro3. (D-R) Immunofluorescence of neural tube sections. Genotypes of the embryos and antibodies as indicated in each panel. Sections in D-O are aligned ventrally (bottom). Sections in P-R are aligned dorsally (top). (S) Quantification of Foxa2⁺ cells in wild type, Hhip1^-/- and Hhip1^-/-;Ptch1^+/- embryos (^*wild type significantly different from Hhip1^-/-;Ptch1^+/-, P<0.08). (T) Quantification of the size of each progenitor domain along the DV axis in cell diameters in wild-type, Hhip1^-/- and Hhip1^-/-;Ptch1^+/- embryos. d, dorsal domain. (U) Representation of the size of each progenitor domain along the DV axis as a percentage of the total DV length of the neural tube. Scale bar: 50 µm.

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