(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 7. Partial isolation of the site of incipient leaf formation (I1),
or of the youngest primordium (P1), from the meristem by a
superficial incision leads to defects in dorsoventral patterning of the
isolated primordium. (A) Tomato apex immediately after isolation of
I1 by an ablation of the superficial L1 layer (black
arrowheads). (B) Semi-thin section of a tomato apex immediately after
isolation of P1 by an ablation as in A (black arrowhead). (C)
Tomato apex 8 days after isolation of P1 as described in A.
P1 lacks any sign of leaflets or of a developing leaf blade. The
trichomes exhibit only abaxial features (compare with
Fig. 5A). (D-I) Tomato apices 8
days after operation as in A. (D) P1 lacks leaflets but has
developed a leaf blade (white arrowheads). (E) Close up view of D. Note the
lack of leaflets in P1, compared with I1 (white
arrowheads). (F) P1 exhibits only one pair of leaflets, compared
with I1, which has already formed two pairs of leaflets (white
arrowheads). (G) I1 is retarded and lacks any sign of dorsoventral
patterning. (H) Completely radialized I1 of approximately normal
size. (I) Initiation of an accessory meristem (white arrowhead) above the
operated I1 position. P4, P3 and
P2 indicate the bases of pre-existing leaf primordia that were
removed at the beginning of the experiment, and P1 represents the
youngest primordium; I1 indicates the first primordium formed after
the ablation. Scale bar: 200 µm.