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Fig. 7. Partial isolation of the site of incipient leaf formation (I1), or of the youngest primordium (P1), from the meristem by a superficial incision leads to defects in dorsoventral patterning of the isolated primordium. (A) Tomato apex immediately after isolation of I1 by an ablation of the superficial L1 layer (black arrowheads). (B) Semi-thin section of a tomato apex immediately after isolation of P1 by an ablation as in A (black arrowhead). (C) Tomato apex 8 days after isolation of P1 as described in A. P1 lacks any sign of leaflets or of a developing leaf blade. The trichomes exhibit only abaxial features (compare with Fig. 5A). (D-I) Tomato apices 8 days after operation as in A. (D) P1 lacks leaflets but has developed a leaf blade (white arrowheads). (E) Close up view of D. Note the lack of leaflets in P1, compared with I1 (white arrowheads). (F) P1 exhibits only one pair of leaflets, compared with I1, which has already formed two pairs of leaflets (white arrowheads). (G) I1 is retarded and lacks any sign of dorsoventral patterning. (H) Completely radialized I1 of approximately normal size. (I) Initiation of an accessory meristem (white arrowhead) above the operated I1 position. P4, P3 and P2 indicate the bases of pre-existing leaf primordia that were removed at the beginning of the experiment, and P1 represents the youngest primordium; I1 indicates the first primordium formed after the ablation. Scale bar: 200 µm.





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