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Fig. 2. {alpha}vß8 integrin is expressed on glial cell processes in the embryonic brain. Horizontal sections through the diencephalon of E13.5 {alpha}v+/- (A-C) or {alpha}v-/- embryos (D-F) labeled with pre-absorbed specific anti-{alpha}v (A,D) and anti-nestin antibodies (B,E). (C,F) Merged images. {alpha}v integrin and nestin expression co-localize on neuroepithelial cell processes in {alpha}v+/- embryonic brain sections (C). No {alpha}v immunoreactivity is present on neuroepithelial cell processes from {alpha}v-/- brain sections (D,F). Horizontal sections through the diencephalon of E11.5 {alpha}v+/- (G-I) or {alpha}v-/- embryos (J-L) labeled with anti-ß8 polyclonal antibody (G,J) and anti-nestin antibodies (H,K). (I,L) Merged images. ß8-integrin and nestin expression co-localize on neuroepithelial cell processes (arrows) in {alpha}v+/- embryonic brain sections (I). ß8 immunoreactivity is not detectable on neuroepithelial cell processes of {alpha}v-/- brain sections, but localizes to neuroepithelial cell bodies (arrowheads; J,L). (M,N) Specificity of the affinity-purified anti-ß8 polyclonal antibody is shown using protein lysates prepared from untransfected COS cells (M, lane 1), or from COS cells transfected with cDNA encoding ß8 (M, lane 2). (N) The anti-ß8 antibody immunoprecipitates cell-surface-labeled {alpha}vß8 from wild-type cultured astrocytes (lane 1), but not from {alpha}v-/- astrocytes (lane 2).





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