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Fig. 6. Chx10 lies downstream of FGF in mouse neuroretinal cell identity decisions. (A) An OV culture from a wild-type animal (+/+) dissected at E9-9.5 and grown for 3 days in the presence of a bovine serum albumin-coated bead (BSA) results in normal development of the NR (arrowhead) and RPE (arrow), including pigmentation and expression of Mitf protein (green). (B) By contrast, a culture of wild-type OV in the presence of an FGF2-coated bead results in a change in cell identity from an RPE to a NR (arrow), as evidenced by a loss of pigmentation and greatly reduced Mitf expression, although the NR appears normal (arrowhead). (C) A Chx10^or-J/or-J OV cultured with an FGF2-coated bead has no effect on the identity of the RPE, as shown by the pigmentation of the RPE and the expression of Mitf (arrow). The ectopic Mitf is clearly apparent in the Chx10^or-J/or-J NR (arrowhead). (D) Graphical representation of the percent frequency of normal RPE pigmentation in optic vesicle cultures incubated with beads coated in BSA, FGF1 or FGF2. Genotype is indicated by bar color (black, wild-type; grey, Chx10^or-J/+; white, Chx10^or-J/or-J). The asterisks represent experiments that were not performed, rather than a value of zero. The numbers above the bars indicate the number of individuals tested. RPE pigmentation: +/+ BSA, 10/10; +/+ FGF2, 1/8; J/J FGF2, 23/29; J/J BSA, 10/10; J/J FGF1, 9/10; J/+ FGF2, 4/28. Scale bars: 20 µm in A-C.

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