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Fig. 2. Identification of hepatocytes and endothelial cells in control mice. (A,B) Liver sections from a chimeric control (A) and a negative control mouse (B) showing DAPI stained nuclei (blue) and YFP fluorescence (yellow). Scale bar: 50 µm. (C-H) Frozen liver section from a chimeric control mouse showing hepatic sinusoids: YFP (yellow); CD31 (red); CD45 (purple); DAPI (blue). YFP+ hepatocytes are outlined with a broken line, arrowheads highlight two representative YFP+ endothelial cells; arrow shows the position of one hematopoietic cell close to a vessel. Scale bar: 25 µm. (I-K) Frozen liver sections from a negative control mouse showing part of a larger hepatic vessel: autofluorescence (yellow); CD31 (red); CD45 (purple); DAPI (blue). The position of the autofluorescent basement membrane is indicated by broken lines. Arrows and arrowheads in I-K indicate the positions of hematopoietic cells and the endothelial cell layer, respectively. Scale bar: 25 µm.





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