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Fig. 5. Analysis of hepatic endothelial cells in vav ancestry mice. (A-C) Frozen section showing sinusoidal endothelial cells (CD31, red), YFP fluorescence (yellow) and hematopoietic cells (CD45, purple). Scale bar: 25 µm. (D-G) Magnification of the area boxed in A-C, showing an YFP^- endothelial cell (arrowhead) and two YFP⁺ hematopoietic cells (arrows) that are closely associated with the sinusoids. DAPI (blue), CD31 (red), YFP (yellow) and CD45 (purple). Scale bar: 10 µm. (H-J) Area with a large hepatic vessel visualized by CD31 staining (red). YFP (yellow), CD45 (purple). Hematopoietic cells closely line the vessel. Scale bar: 25 µm. (K-N) Magnification of the area boxed in H-J showing two YFP^- endothelial cells (arrowheads) integrated into the vessel wall and one YFP⁺ hematopoietic cell on the abluminal side of the endothelial lining: DAPI (blue); CD31 (red); CD45 (purple). Scale bar: 10 µm.

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