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Fig. 6. FACS analysis of liver cell suspensions. (A-C) Gating logic for cells in organ cell suspension based on exclusion of dead cells by DAPI staining (A) and doublets by pulse width analysis (B). (C) Endothelial cells (EC) and hematopoietic cells (HC) are defined by marker gene expression. (D) YFP labeling of CD45+ hematopoietic cells isolated from the liver of a vav ancestry mouse. (E-H) YFP labeling of hepatic endothelial cells from a negative control mouse (E), a vav ancestry mouse (F), a chimeric control mouse (G) and a wild-type mouse completely reconstituted (>95%) with bone marrow of a vav ancestry mouse three months after transplantation (H).





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