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Fig. 4. The posterior limit of expression of AmphiNodal (A-L) and
AmphiOtx (M-R) in the endoderm is not affected by altered levels of
RA signaling until the late neurula/early larval stage. Anterior to left.
Whole mounts viewed from the left side and frontal sections in B,F,J viewed
from dorsal side. The `x' in A,E,I shows the level of the section in B,F,J,
respectively. Arrowheads indicate the posterior limit of expression in the
endoderm. Scale bars=50 µm. (A) At midneurula (16 hours),
AmphiNodal is expressed throughout the length of the endoderm. The
posterior limit of expression in the endoderm at this stage is not affected by
either RA (E) or by BMS009 (I). The frontal sections show that expression of
AmphiNodal is restricted to the left side of the pharynx in controls
(B), RA-treated (F), and BMS009-treated (J) embryos. By the late (24-hour)
neurula, expression of AmphiNodal is restricted to the tail bud and
pharyngeal endoderm in DMSO-treated controls (C), and is shifted slightly
anteriorly by RA (G) and posteriorly by BMS009 (K). In early (30-hour) larvae,
pharyngeal expression of AmphiNodal is reduced in the pharynx of
controls (D), eliminated in RA-treated larvae (H) and expanded posteriorly in
animals treated with BMS009 (L). (M) At the late (20-hour) neurula,
AmphiOtx is expressed throughout the length of the pharynx in
DMSO-treated controls. At this stage, the posterior limit of AmphiOtx
expression is not affected by either RA (O) or BMS009 (Q). In 30-hour larvae,
endodermal expression of AmphiOtx is restricted to the pharyngeal
endoderm in DMSO-treated controls (N) and its posterior limit is shifted
anteriorly by RA (P) and posteriorly by BMS009 (R).
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