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Fig. 6. Knockdown of AmphiHox1 function with an antisense morpholino
oligonucleotide mimics the effect of BMS009 treatments on the posterior limit
of the pharynx in amphioxus. Scale bar=50 µm. (A,C,E,G) Embryos injected
with the control morpholino. (B,D,F,H) Embryos injected with the
AmphiHox1 antisense morpholino-oligonucleotide. (A,B)
AmphiHox1 expression. In controls (A), the anterior limit of
AmphiHox1 expression in the endoderm (arrowhead) coincides with the
first pigment spot to form in the nerve cord, and is shifted slightly
posteriorly in embryos injected with the AmphiHox1 morpholino (B).
(C,D) AmphiPax1/9 expression. The posterior limit of
AmphiPax1/9 expression in the endoderm (arrowhead) of an amphioxus
larva injected with the control morpholino (C) coincides with the first
pigment spot to form in the nerve cord (double arrowhead), whereas in larvae
injected with the AmphiHox1-specific morpholino (D), the pharynx is
expanded posteriorly and the posterior limit of AmphiPax1/9
expression in the pharyngeal endoderm (arrowhead) is posterior to the first
pigment spot in the nerve cord (double arrowhead). (E-H) AmphiOtx
expression. In control embryos (E,G), the posterior limit of AmphiOtx
expression (arrowhead) coincides with the first pigment spot in the nerve cord
(double arrowhead). AmphiOtx expression is expanded posteriorly in
embryos injected with the AmphiHox1-specific morpholino (F,H).
A,B,E,F=30 hours. C,D,G,H=40 hours.
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