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Fig. 1. Sequence analysis and phylogeny of the new bHLH transcription factor Him.
(A) Genomic organization of the him/her5 locus. The coding
region is interrupted by three introns, the bHLH domain being encoded by exons
E2 and E3. Note that the first exon of him is within the domain
identified as necessary for MH expression of her5
(Tallafuss and Bally-Cuif,
2003). (B) Phylogenetic tree of the zebrafish Her family, showing
relationship between known and predicted Her proteins. The alignment used for
tree construction was obtained using the VectorNTI software, and is based on
full-length sequences. Paired genes are boxed in blue, and individual pairs
are marked with the same symbol (oval, rectangle or diamond). Accession
numbers for the proteins used in this tree are: Her5 (NP_571152),
Her1(NP_571153), Her7(NP_571684), Her4(NP_571165), Her2(NP_571164),
Her3(NP_571155), Her9(NP_571948) and Her6(NP_571154). Proteins predicted by
The Wellcome Trust Sanger Institute
(http:www.ensembl.org)
are: Her11, Her12, Her13 and Hes6. (C) Sequence alignment of Her1, Her5 and
Him. Basic domains are overlaid with red asterisks, Helix 1 domains with dark
blue, loop domains with green, Helix 2 domains with light blue, orange domains
(H3/H4) with orange stars and WRPW tetrapeptides
