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Fig. 6. The activity of both Her5 and Him is necessary to prevent neurogenesis in the lateral IZ. Dorsal views of flat-mounted -8.4ngn1::egfp transgenic embryos (Blader et al., 2003) probed at the three-somite stage for egfp expression (blue) after injection of 1 mM her5MOATG + 0.25 mM himGripNAATG (A), 1 mM her5MOATG (B), 0.25 mM himGripNAATG (C), compared to an uninjected control (D). A' and D' are enlargements of the boxed areas in A and D, respectively. The IZ is indicated by pax2.1 expression (red staining). Note that the LIZ is undergoing ectopic neurogenesis only in the embryo injected with both her5MOATG and himGripNAATG (blue arrows).





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