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Fig. 5. The ETO region of AML1-ETO is required for activity. Larval eye discs are stained for AML1 (red), ß-gal (green) and Elav (blue). (A,B) Wild-type eye (A) and larval eye disc (B), showing the normal expression pattern of SME-lacZ. anti-AML1 does not recognize any of the endogenous fly RD proteins. (C,D) lz-Gal4 UAS-NLS-AML1{Delta}ETO eyes appear wild type (C) and expression of NLS-AML1{Delta}ETO does not affect SME-lacZ expression or eye disc development (D). (D, inset) An ommatidium is enlarged to show that NLS-AML1{Delta}ETO colocalizes with Elav in the nucleus.





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