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Fig. 6. Loss-of-function and gain-of-function of scrb1 confirm that scrb1 is homologous to the llk gene. (A-C) Embryos injected with MO did not show any migration of the nVII motor neurons. MOs were designed to disrupt the translation (B, MO/ATG) or splicing (C, MO/2e2i) of the scrb1 mRNAs (compare with the wild-type embryo shown in A). Dorsal views, 2 dpf. (D-G) Structure-function analyses of Scrb1. Wild-type and mutated scrb1 mRNAs (schematically drawn in D) were injected into llk^rw468 embryos. Injection of wild-type scrb1 mRNA restored migration of the nVII motor neurons (F, compare with control llk^rw468 embryo shown in E). Injection of scrb1^rw16 mRNA also restored the migration (G) although at lower frequency. (H-K) Subcellular localization of wild-type and mutated Scrb1 proteins. Scrb1, Scrb1^rw16 and Scrb1^PDZs are associated with plasma membranes (H,I,K). However, Scrb1^rw468 failed to localize to membranes (J). A-C, E-G, dorsal views, 2 dpf. H-K, 10-12 hpf. Scale bars: 50 µm (A-G) and 20 µm (H-K).

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