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Fig. 2. S-phase entry in the secondary and tertiary precursor cells. BrdU
incorporation (red) in A101 pupae at 17 hours APF during pI division (A-B) and
in pupae at 19 hours APF during pIIb division (C-D). Bristle lineage cells
were identified by specific anti-ß-gal or anti-Sens immunoreactivity
(blue). Cells in telophase were identified by incomplete formation of the
nuclear envelope, as suggested by lamin immunoreactivity in the cytoplasm (A)
and by the presence of the midbody (green) detected by 
-Tub
immunoreactivity (B,C). Note in C that that midbody is observed along the axis
of division that follows an apico-basal orientation. In D, interphasic cells
showing well-defined Lamin-labelling (green) around the nuclei. In each panel,
overlay is on the right. Note in B that BrdU incorporation takes place in pIIa
and pIIb cells, while the ß-gal was still present in the cytoplasm. Note
also that there is no BrdU incorporation late in pIIb telophase (C) and after
formation of the pIIIb nucleus (D). Scale bar: 2 µm. g, glial cell.
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