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Fig. 5. The activity of CycE controls the G1 phase in secondary and tertiary
precursor cells. (A) dap4 null mutant pupae at 19 hours
APF, BrdU incorporation (red) occurs during pIIb telophase, identified by the
presence of the midbody (
-Tub, green). (B) After overexpression of
CycE, BrdU incorporation (red) occurs during pIIb telophase, revealed by the
midbody staining (-Tub; green). Note that the BrdU-positive cell is the
larger of the pair, suggesting that it corresponds to the pIIIb cell. Nota
from neuP72> CycE pupae at 19 hours APF. (C)
Overexpression of CycE induces precocious BrdU incorporation (red) in the
shaft and the socket cells. Nota from neuP72> CycE
pupae at 19 hours 30 minutes APF. Note that simultaneous BrdU incorporation in
the pIIIb, the shaft and the socket cells was never observed in control
animals. (D) Overexpression of Dap triggered a G1-phase in pIIa and pIIb
cells. By contrast to control animals (see
Fig. 2A), no incorporation of
BrdU (red) was observed during pI telophase, identified by the presence of the
midbody (-Tub; green). Nota from neuP72> Dap
pupae around 17-18 hours APF. In all panels, sensory organ cells are
identified by anti-Sens immunoreactivity (blue in A,B,D and green in C) and
the overlay is on the right. g, glial cell; sh, shaft cell; so, socket cell.
Scale bar: 2 µm.
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