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Fig. 6. Ttk69 repressed CycE expression in pIIa cells. (A-C) Expresssion pattern of
Ttk in the bristle cell lineage. Ttk immunoreactivity (red) of nota from A101
pupae between 16 and 24 hours APF. Sensory organs were identified with anti
ß-gal antibodies (green). Note that Ttk expression preceded pIIa cell
division. (D) Ttk overexpression repressed CycE expression in pIIb cells. A
sensory organ from hs-Gal4>Ttk69 pupae. CycE detection is in red and Sens
in green. Note that in three-cell clusters, CycE was not present in pIIIb
cells (compare with control animals, Fig.
4F,T). (E-G) ttk1e11 somatic clones. Clones
were detected by the lack of GFP staining (green), their limits are shown with
a white dotted line. Sensory organs were detected with anti-Cut (E,F) and
anti-Sens (G) antibodies (green). (E) Ttk69 protein is not detected in
clusters in ttk1e11 somatic clones. Ttk protein is
detected in red. Nota from pupae at 23 hours APF. (F) Socket cells are present
in sensory organs inside ttk1e11 clones. Socket cells are
identified by the high expression of Su(H) (red). Nota from pupae at 23 hours
APF. In E and F, arrows indicate clusters located in the clone. (G) Ectopic
CycE expression was observed in the progeny of pIIa cells in
ttk1e11 somatic clones. Each panel is shown separately in
G', G", G"'. (G') ttk1e11 somatic clones
identified by the lack of GFP staining, outlined by a white dotted line.
Sensory organs were detected with anti-Sens antibodies. (G") Prospero
expression revealed the pIIb progeny (arrowheads). (G"') CycE
accumulates in all cells of clusters inside the clone. Note that even pIIa
daughter cells, identified by the lack of prospero staining (arrows), are CycE
positive. Nota from pupae around 21 hours APF. g, glial cell; s, sheath cell;
n, neuron; sh, shaft cell; so, socket cell. (A-D) Scale bars: 2 µm in A-D;
10 µm in E-G.
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