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Fig. 3. Gene expression analyses of Gbx2 mutants. Whole mounts of
heterozygous (A) and homozygous (B) Gbx2 embryos probed for
Wnt2b transcripts at 10 dpc. Arrows indicate the presence or absence
of Wnt2b expression in the otocyst. An arrowhead indicates
Wnt2b expression in the eye. Inserts in A and B show +/– (A)
and –/– (B) otocysts at 9.5 dpc that have undergone prolonged
color development. (C-F) Cryosections probed for Dlx5 (C,D) and
Hmx3 (E,F) transcripts at 9.5 dpc. Pairs of 12 µm adjacent
sections from +/– (C,E) and –/– (D,F) Gbx2 inner
ear. (C) Dlx5 is expressed in the entire dorsal region of a +/–
otocyst, whereas Hmx3 is expressed only in the lateral region (E,
arrows). (D,F) In a –/– otocyst, the medial expression domain of
Dlx5 is absent, whereas both Dlx5 and Hmx3 are
expressed in the lateral regions. Arrows in D-F indicate the borders of
expression domains. Scale bars: in B, 100 µm for A,B; in D, 50 µm for
C-F.
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