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Fig. 5. Sensory organ formation in Gbx2^-/- inner ears. Sections of heterozygous (A,C,D,G,H) and homozygous (B,E,F,I,J) Gbx2 inner ears probed for Lfng or Myo15a transcripts at 10.5 (A,B) and 15.5 (C-J) dpc. (C-J) Pairs of 12 µm adjacent sections. Red lines indicate comparable Lfng expression domains between +/– and –/– ears. Lfng (E) and Myo15a (F) are expressed in the lateral crista (lc) and macula utriculi (mu) of a Type IV Gbx2^-/- inner ear. Arrowheads in H indicate the sensory hair cells. Only one or two Lfng- (I) and Myo15a (J)-positive sensory patches are present in a Type II Gbx2^-/- cochlear duct. Insert in J shows a higher power view of the Myo15a positive sensory region. Arrowheads indicate the region that is positive for both Lfng and Myo15a. Scale bars: in A, 100 µm for A,B; in C, 100 µm for C-F; in G, 100 µm for G-J.

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