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Fig. 1. A method for attenuating Bmp signaling in transgenic zebrafish. Diagram of
a wild-type cell receiving a Bmp signal (left) and a transgenic cell receiving
a Bmp signal after heatshock (right). In a wild-type cell, the Bmp ligand
binds to a Type I/Type II receptor complex, which then phosphorylates and
activates a receptor SMAD (rSMAD), leading to target gene activation. After
heatshock of the transgenic cell, the truncated Type I Bmp receptor containing
GFP in place of the kinase domain displaces the endogenous Type I receptor,
preventing the receptor complex from activating rSMADs. As a result,
downstream target genes are not activated, and the cell is reduced in its
capacity to respond to Bmp signals. KD, kinase domain; LB, ligand-binding
domain; P, phosphate; TM, transmembrane domain.
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