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Fig. 2. Phenotypes caused by reducing Bmp signaling at various developmental
stages. Embryos from a transgenic outcross were heatshocked in an air
incubator at 3 hpf (A-C) or shield stage (D-F), and the GFP-positive embryos
were sorted and scored for dorsalized phenotypes at 30 hpf. Note the fully
formed tail in the wild-type sibling (whole embryo in A, tail view in D).
Transgenic embryos heatshocked at 3 hpf display both C4 (B) and C3 dorsalized
(C) phenotypes. Note the curled trunk and tail in B and the curled tail in C.
Transgenic embryos heatshocked at shield stage display either a C1 phenotype
(E; arrow denotes loss of the ventral tail fin) or a C1 phenotype coupled with
the formation of an ectopic tail (F; arrowhead denotes second tail). ET,
ectopic tail.
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