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Fig. 3. eve1 expression is dependent on Bmp signaling both during and
after gastrulation. Embryos were heatshocked in an air incubator at the
indicated stages, and the embryos containing the transgene were sorted by GFP
fluorescence. (A,B) Vegetal views of embryos heatshocked at shield stage and
assayed for eve1 expression at 70% epiboly. Note the light staining
on the left (ventral) side of the transgenic embryo (B) compared with the
wild-type sibling (A). (C,D) Posterior views of embryos heatshocked at bud
stage and assayed for eve1 expression at the 7-somite stage. Note the
darker staining of eve1 in the wild-type embryo (C), with expression
extending farther anteriorly than in the transgenic embryo (D). (E,F)
Posterior views of embryos heatshocked at the 3-somite stage, then assayed for
eve1 expression at the 9-somite stage. Note the darker staining of
eve1 in the wild-type embryo (E) than in the transgenic embryo (F).
Insets in C-F show lateral views of the tailbud. HS, heatshock.
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