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Fig. 4. High levels of Bmp signaling are important for ventral mesoderm patterning
during gastrulation but not afterward. Embryos were heatshocked in an air
incubator at 3 hpf for 1 hour, or heatshocked continuously from bud stage,
sorted for GFP fluorescence and fixed at the 12-somite stage. Expression of
flk-1 (A-C), gata1 (D-F) or pax2.1 (G-I) was then
examined by in-situ hybridization. Non-transgenic embryos heatshocked under
either protocol were identical, so only a single example is shown for the
–GFP samples (C,F,I). Expression of all three markers was reduced in
transgenic embryos (A,D,G) compared with wild-type siblings (C,F,I) after 3
hpf heatshocks. Gene expression was unaltered following post-gastrula
heatshocks (B,E,H). Arrows in A-C denote flk-1-expressing cells.
Arrowheads in G-I denote pax2.1 expression in the otic placode, while
brackets indicate pax2.1 expression in the pronephric ducts and
tubules. Views in A-C and G-I are dorsal, with anterior to the top. (D-F)
Posterior views of embryos.
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