|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Analysis of gene expression in the ectopic tails. Embryos were heatshocked
from shield to bud stage to maximize ectopic tail formation, then photographed
live at 3 dpf (A), or fixed at either 21 hpf (B) or 30 hpf (C-I). Expression
of eve1 (B,C), no tail (ntl; D), myod (E),
collagen 2a (coll2a; F), caudal (G),
neurogenin1 (H), or sonic hedgehog (shh; I). Arrows
in each panel indicate the location of the ectopic tail. In A note the
presence of both fin and pigment tissue in the secondary tail. In all other
panels except H note the expression of each corresponding gene in the ectopic
tails. ntl expression in D was localized only to the tailbud of the
ectopic tail. All images except I are representative of the majority of
ectopic tails examined. We observed sonic hedgehog-expressing cells
in 44% (n=16) of ectopic tails.
|
