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Fig. 6. Dynamic growth of secondary tails. Confocal microscopic examination of live
truncated Bmp receptor-GFP/tbx6-gfp transgenic embryos during
secondary tail growth. (A) Time points were started at the 25-somite stage,
then taken every hour afterwards, corresponding to approximately one somite
per time point at room temperature. Note the growth of the GFP-expressing
secondary tail and the maturation of the secondary tail's presomitic mesoderm
into somitic blocks. Arrows indicate the second tail, which grows along the
primary axis. The asterisk indicates the first fully formed ectopic tail
somite at the 25-somite stage. (B) In a separate experiment, truncated Bmp
receptor-GFP/tbx6-gfp embryos were mounted at the 16-17-somite stage,
then monitored by confocal microscopy to detect the emergence of a second
tail. Confocal slices from two representative embryos are shown. Arrows denote
the tip of the second tailbud, and `ye' indicates the yolk extension. In a and
c, note the ventral patch of GFP expression corresponding to an emerging
second tail. (b,d) The extending second tail about 4 hours later. The ectopic
tail is growing into the plane of the picture in b and out of the plane in
d.
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