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Fig. 7. Only cells with reduced Bmp signaling can populate ectopic tails. (A)
Diagram of the transplant scheme used. Fluorescein-labeled donor cells (wild
type or transgenic) were transplanted at dome stage to the ventral side of
shield-stage transgenic hosts. Hosts were immediately heatshocked, then
analyzed at 24 hpf (after the transgenic GFP faded) for presence of
fluorescent cells in the ectopic tails. (B) Wild-type donor cells in a
transgenic host. Note the absence of fluorescein-labeled donor cells in the
ectopic tail (denoted by an arrow). (C) Transgenic donor cells in a transgenic
host. Note the presence of fluorescein-labeled donor cells in the ectopic tail
(denoted by an arrow) as well as in the primary tail.
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