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Fig. 4. Production of grk in nurse cells is sufficient to establish the oocyte DV axis. (A) In wild-type egg chambers, mirr-lacZ is expressed in dorsal anterior follicle cells in response to Grk-Egfr signaling. (B) mirr-lacZ is not expressed in egg chambers with grk^2B6/grk^2B6 germline clones, confirming that this cell fate marker is grk-dependent. (Note that the group of small GFP-positive cells is the migrating border cell cluster.) (C) Two focal planes of a germline mosaic egg chamber with a homozygous wild-type oocyte. mirr-lacZ is expressed in dorsal anterior follicle cells. (D) Two focal planes of a germline mosaic egg chamber with a grk^2B6/grk^2B6 oocyte; arrows indicate a single nucleus that is visible in both images. This egg chamber is slightly rotated laterally with respect to that shown in C, accounting for the minor apparent difference in expression pattern. There was no significant difference in the mean (±s.d.) number of mirr-lacZ-positive nuclei between germline mosaics with a mutant oocyte (165±46) and either mosaics with wild-type oocyte (205±43) or grk heterozygotes (158±55).

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