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Fig. 5. Production of grk in the oocyte is not required for induction of dorsal follicle cell fates. Dorsal view of the BR-C expression pattern in germline mosaics with (A) a wild-type oocyte (confocal image) or (B) a grk^2B6/grk^2B6 oocyte. Measurement of the dorsal midline domain that lacks BR-C expression revealed that the mean (±s.d.) dimensions of this domain were 11.3 (±1.3) cells along the AP axis and 3.4 (±0.7) cells along the DV axis (n=15) in mosaics with a wild-type oocyte, 10.2 (±1.0) cells along the AP axis and 2.3 (±0.6) cells along the DV axis (n=12) in mosaics with a grk mutant oocyte, and 10.9 (±1.6) cells along the AP axis and 3.3 (±0.7) cells along the DV axis in grk heterozygotes (not shown). Although this domain is approximately one cell shorter along both axes in mosaics with a grk mutant oocyte, the resolution of this assay is limited by the dynamic nature of the BR-C expression pattern, as reflected in the range observed in grk heterozygotes.

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