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Fig. 6. Nurse-cell-derived grk transcripts generate properly localized Grk. (A,B) Grk protein is properly localized in nuclear GFP-marked germline mosaics with either a wild-type (A, confocal image) or grk2B6/grk2B6 (B) oocyte. The arrow in B indicates a nurse cell nucleus with high levels of GFP that lies behind the focal plane shown. (C) Dorsal view of a germline mosaic with the lacO/GFP-LacI genotypic marker. The oocyte nucleus exhibits two foci of GFP fluorescence (inset), while four nurse cell nuclei lack foci (outlined in blue; the presence or absence of foci was determined by inspection of individual confocal sections at the appropriate exposures). Although this oocyte is homozygous for the grk2B6 allele, Grk (red) exhibits a normal dorsal anterior localization typical of this stage.





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