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Fig. 5. Co-activation of Pax3 and Zic1 in concert with Wnt
signaling is essential for neural crest determination of the ectoderm in vivo.
(A-F) Synthetic mRNA was injected into a ventral animal blastomere at the
eight-cell stage. Embryos were harvested at stage 15 for in situ hybridization
with Foxd3 (A,C,E) or Slug (B,D,F) probes. A higher
magnification view is shown in the right half of each panel. Pax3 and
Zic1 mRNA injection with lacZ mRNA (A-F; ß-gal activity
was visualized by incubating with Red-Gal), ß-catenin-MO (Gene
Tools; 10 ng/cell; C-F), and ß-catenin (50 pg of DNA/cell;
E,F).
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