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Fig. 2. PAT-related transporters phenocopy the effects of TOR on growth and cell number in the wing. (A-H) Expressing transgenes under the control of MS1096-GAL4, which drives transcription predominantly on the dorsal surface of the wing, resulted in an overall increase in wing size for CG1139^GS10666 (D), but a reduction for path^GS13857(C), Tor (E) and UAS-CG1139 (F). Overexpression of slif had no significant effect (B) relative to control wings (A). Both path^GS13857 (G) and CG1139^GS10666 (H) significantly enhanced the phenotype produced by overexpressing Tor. Co-expression of two CG1139 GS insertions (CG1139^GS10666 and CG1139^GS7120) inhibited growth (I) and CG1139^GS10666 enhanced the growth inhibitory effect of UAS-CG1139 (J). (K-M) Histograms showing measurements of posterior compartment size (ratio of a specific wing area expressing the transgene and a non-expressing region=P/A) (K), cell size (L) and cell number (M) taken from adult female wings expressing transgenes under en-GAL4 control. Expression of each PAT-related transporter (two GS insertions and a UAS-transgene), TOR or a dominant-negative form of TOR (TOR-TED), but not Slif, produced highly significant changes in wing and cell size (P<0.001). Cell number was significantly reduced upon overexpression of TOR, TOR-TED and those PAT-related transporter constructs that have the greatest effects on growth (^*P<0.01; ^**P<0.001).

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