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Fig. 5. Cellular basis of the cv-c phenotype. (A-D) Planar polarity of the
dorsal epidermis is unaffected in cv-c embryos. In wild-type embryos,
the Cno protein (red) is initially expressed around the entire cell cortex,
but later clears from sites of apposition with the amnioserosa and refines to
distinct puncta (arrowheads), where adjacent epidermal cells meet. This
pattern is seen in cv-c embryos (B,D) as in wild type (A,C). (E-H)
Apicobasal polarity in the MpTs is unaffected in cv-c embryos.
Embryos in which UAS-CD8-GFP was driven in the MpTs to label the entire
membrane (green) were also stained with Baz (red) to mark the apical membrane.
Wild-type (E,F) and cv-c (G,H) at stages 13 and 16. In cv-c
tubules, apicobasal polarity is established and maintained. (I,J) The adherens
junctions appear unaffected in cv-c embryos. Wild-type (I) and
cv-c (J) stage 14 embryos stained with Sas (green) and Ecad (red).
The level of expression and the relative position of the two markers in
cv-c MpTs is identical to wild type. Asterisk in J indicates the
lumen. (K-P) The level and distribution of cortical F-actin is disrupted in
the MpTs of cv-c embryos. (K) In wild-type stage 13 MpTs, F-actin is
localised to both the lateral and, in particular, the apical cell cortices.
(L,M) F-actin continues to accumulate at the lateral and apical cell cortices;
at stage 14/15, it appears compact and in close apposition to the plasma
membrane (inset in L). (N) In stage 13 cv-c mutant MpTs, F-actin
fails to accumulate in the cortex, remaining diffuse in the cytoplasm. (O) In
stage 14 cv-c mutant MpTs, F-actin remains perinuclear, showing
little concentration in the subcortex (inset in O). (P) In a small number of
cv-c mutant MpTs, the distal end of the tubule elongates (arrowhead);
in these cases, cortical F-actin is similar to wild type (M) seen at stage
16.
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