DEV01793 Supplementary Material

Files in this Data Supplement:

• Supplemental Table (Adobe PDF)
• Supplemental Figure 1 -

  Fig. S1. Loss of wild-type patched expression in pre-neoplastic and tumor cells. RNA was purified from FACS-sorted (GFP⁺) GCPs, pre-neoplastic cells and tumor cells from Math1-GFP/patched^+/– mice, and from intact cerebellum of adult patched^+/– mice. RNA was reverse transcribed and subjected to real-time PCR analysis in triplicate using exon-specific primers for patched (see Fig. 7). Mean expression levels were normalized to actin and raw data from individual samples is presented. Six independent experiments confirmed loss of wild-type patched expression in a total of six out of seven pre-neoplastic samples and 13 out of 13 tumor samples.

• Supplemental Figure 2 -

  Fig. S2. Validation of microarray data using real time RT-PCR. RNA was purified from three independent samples of GCPs, pre-neoplastic cells and tumor cells. RNA was reverse transcribed and subjected to real-time PCR analysis using primers for the genes indicated. Expression levels were normalized to actin and divided by the levels in GCPs to calculate fold change. Data represent means of three samples of each cell type in triplicate ±s.e.m.