Supplemental Figure 1
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Fig. S1. Genital
tubercles cultured with flutamide, an androgen receptor antagonist, develop
dose-dependent hypospadias. (A-E)
Scanning electron micrographs showing ventral side of genital tubercles after
48 hours in organ culture with flutamide at concentrations of 10-5 M
(B), 10-4 M (C), 5-4 M (D) and 10-3 M (E). (A)
Control genital tubercle cultured with ethanol but without flutamide has
undergone normal morphogenesis. (F,G) Histological sections through genital
tubercles cultured for 48 hours. Control ethanol-treated culture (F) shows
stratified urethral plate epithelium (box), whereas genital tubercle cultured
with 5-4 M flutamide (G) shows hypoplastic, disorganized urethral
plate (box).
