|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Expression Fgfr2-IIIb and Fgf10 in urethral epithelium is
regulated by the androgen receptor. (A-F) Ventral views of whole genital
tubercles from male embryos cultured for 48 hours and processed for
whole-mount in situ hybridization with a Fgfr2-IIIb riboprobe. Arrows
indicate the position of urethral plate epithelium. (A) Fgfr2-IIIb
expression in the urethral epithelium of 48 hour control culture. (B-E)
Fgfr2-IIIb expression in genital tubercles cultured with the androgen
receptor antagonist flutamide at concentrations of 10-5 M (B),
10-4 M (C), 5-4 M (D) and 10-3 M (E). There
is a dose-dependent decrease in Fgfr2-IIIb expression, hypoplasia of
the prepuce and malformation of the glans. (F) Fgfr2-IIIb expression
and normal morphology is rescued in urethral plate of genital tubercle treated
with flutamide (5-4 M) by addition of dihydrotestosterone (DHT;
5-6 M) (compare F with D). (G) Partial sequence of Fgfr2
promoter (nucleotides 1041-1610) showing the presence of stereotypical
androgen response element (ARE) in red. (H-J) Ventral views of female genital
tubercles cultured for 48 hours and probed for expression of Fgf10.
(H) Control culture. (I,J) Fgf10 expression persists in mesenchyme of
the glans after treatment with 10-5 M flutamide (I), but is
undetectable after treatment with 10-3 M flutamide (J). Arrowheads
in H,I indicate Fgf10 expression in mesenchyme.
|
