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Fig. 3. Altered cardiac transcriptional program in Tbx20lacZ/lacZ embryos. (A-H) Whole-mount in-situ hybridization analysis of E8.25-9.5 wild-type or Tbx20lacZ/lacZ embryos showing expression of genes indicated. Arrows in B indicate cranial limit of Tbx5 expression. Arrows in E indicate cells of the primary heart field. (I) Tbx20lacZ/+ and Tbx20lacZ/lacZ embryos bearing the Mef2c-AHF-hPLAP transgene, stained for alkaline phosphatase activity. oft, outflow tract; wt, wild type.





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