DEV01827 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Efficacy and specificity of Tbx20 shRNAs. (A) Diagram of the protein-coding portions of the Tbx20 cDNA, including the T-box (red) and the activation/repression domain (‘regulatory’, green). Location of the siRNA sequences are indicated by a and b. (B-D) Western blot of cell extracts from 10T1/2 cells co-transfected with mycTbx20;IRES-EGFP (mycTbx20-IE, B), flagTbx5 or Tbx5;IRES-EGFP (Tbx5-IE) (C), or mycTbx1;IRES-EGFP (mycTbx1-IE, D) expression constructs and vectors expressing Tbx20 shRNAs a or b. Protein was probed with anti-myc, anti-flag, or anti-EGFP antibodies. (E-H˘) Immunofluorescent detection of mycTbx20-IE (green in E,F,F˘), Tbx5-IE (green in G,G˘), or mycTbx1-IE (green in H,H˘) and Tbx20 shRNA vectors (by EF1a-dsRed detection, red in E-H˘).

• Supplemental Figure 2 -

  Fig. S2. TUNEL (A-F) and Ki67 (G-J) staining on wild type (A,C,E,G,I) and Tbx20 knockdown (siTbx20; B,D,F,H,J) embryos at E9. C-F and I,J are higher magnification views of A,B and G,H.