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Fig. 2. Whole-mount in situ analysis of Tbx20-null mutants and control littermates. (A-H) Differentiation markers MLC2a, ß-MHC and MLC2v are normally expressed in Tbx20 mutants; {alpha}-MHC, however, is downregulated. (I,J) Tbx5 is expressed in left ventricular, atrioventricular and atrial progenitors in an anteroposterior gradient. This expression profile is maintained in Tbx20 mutants. (K,L) Wnt11 is expressed in outflow tract and atrioventricular canal. In Tbx20 mutants, outflow tract expression is present, but atrioventricular canal expression is lacking. (M,N) GATA4 is expressed throughout the heart in an anteroposterior gradient that is maintained in Tbx20 mutants. Arrows indicate the corresponding heart regions in wild type and mutant embryos.





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