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Fig. 4. VMZ explants co-injected with Tsg-MO and Bmp7-MO are strongly dorsalized and adopt the identity of DMZ explants. (A) Experimental design of the DMZ, LMZ and VMZ explants. (B) VMZs from wild-type embryos cultured until tailbud stage 20. (C) VMZs from Bmp7-MO-injected embryos are dorsalized and resemble LMZs (inset). (D) VMZs from Tsg-MO-injected embryos showing dorsalization (unpigmented regions). (E) VMZs from Tsg-MO- and Bmp7-MO-injected embryos elongate similarly to DMZ (Spemann organizer) explants (shown in the inset). (F) RT-PCR assays of stage 26 whole embryos (WE) (lane 1), DMZs (lane 2), LMZs (lane 3) and VMZs (lane 4) from uninjected embryos or from embryos injected with Tsg-MO (lane 5), Bmp7-MO (lane 9), with Tsg-MO or several doses of Bmp7-MO (lanes 6-8). VMZs from embryos injected with both MOs express the same markers as wild-type DMZ explants (compare lanes 2 and 8), indicating strong dorsalization and reduced Bmp signaling. VP, vegetal pole; WE, whole embryo.





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