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Fig. 6. Onset of ventral and posterior mesoderm defects in
Tsg-/-;Bmp7-/- and
Tsg+/–;Bmp7-/- compound mutants.
(A,A') Ventral view of posterior primitive streak (ps) region of
wild-type and mutant embryos stained with brachyury. The expression of
brachyury is unchanged at 8.25 dpc in the mutant. (B,B') Side view of
8.25 dpc embryos before turning hybridized with Fgf8. Staining of the
primitive streak and posterior mesoderm (pm) is fainter in the mutant.
(C,C') Dorsal view of the posterior region of embryos shown in B and
B', respectively. Fgf8 expression is less intense in the
mutant. (D,D') High magnification view of the tailbud region of embryos
stained with brachyury; in the mutant staining of tailbud mesoderm at the tip
appears fainter, but notochord (n) expression is unaffected. (E,E')
Bmp4 expression in side view of 9.0 dpc embryos (after turning). The
allantois (al) and lateral plate mesoderm (lpm) are stained. (F,F')
Higher magnification view of the tailbud region of embryos hybridized with
Bmp4. The tailbud and of the allantois are abnormal in shape. Upon
dissection, the allantois was found not to be fused to the chorion in the
mutant (chorio-allantoic fusion has normally completed by this stage).
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