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Fig. 1. Morphological features of the developing lymph gland. (A,B) Lymph glands at
embryonic stage 16. Cells of the embryonic lymph gland (shown in whole mount)
express the hemocyte marker Srp (A, red) and Odd (odd-lacZ expression
in B, green; the bluish-green appearance is due to overlap with nuclear marker
To-pro-3 in blue). Pericardial cells (B, arrowhead), also express Odd. Cells
between the two symmetric lymph gland clusters represent dorsal vessel (DV)
cells (B). Scale bar: 10 µm. (C,D) Second instar larval lymph gland.
Expression of Srp (C) and odd-lacZ (D) is maintained in the second
instar. By this stage, the lymph gland consists of a pair of primary (1°)
lobes and smaller, posterior secondary (2°) lobes separated by pericardial
cells (arrowhead in D). Scale bar: 10 µm. Expression of odd-lacZ
is slightly higher in the region of the posterior signaling center (PSC) of
the primary lobes. (E) Multi-lobed structure of the third instar lymph gland.
Srp (red) is ubiquitously expressed. Pericardial cells separate lymph gland
lobes as in the second instar. Scale bar: 100 µm. (F,G) Expression pattern
of Viking-GFP fusion protein (F, green) reveals chamber-like structures within
the lymph gland. Similar structures are seen in lymph glands of the
uncharacterized GFP-trap line ZCL2867 (G, green). Scale bar: 10
µm. To-pro-3 (blue) shows nuclei. (H,I) Morphological features of the third
instar lymph gland. (H) A DIC image of the third instar lymph gland shows two
distinct regions within the primary lobe that we call the cortical zone (CZ)
and the medullary zone (MZ) (see text for details). Arrows indicate the
boundary between the zones. The secondary (2°) lobe is similar in
appearance to the medullary zone. (I) Confocal section through a lymph gland
from the GFP-trap line G147. Cells in the medullary zone (MZ,
outlined) are compactly arranged, whereas cells in the cortical zone (CZ)
exhibit voids and gaps. Scale bar: 10 µm.
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