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Fig. 2. Maturing hemocytes are found in the cortical zone. (A)
Cg-gal4/UAS-GFP-expressing cells (green) are found in the periphery
of the primary lobe. (B,B') Flattened confocal image of a third instar
G147 lymph gland. GFP expression (green) identifies the cortical and
medullary zones. Cg-gal4/UAS-lacZ (B', red) expression is
restricted to the cortical zone (CZ) and is excluded from the medullary zone
(MZ). (C-G) Mature hemocyte markers are restricted to the cortical zone.
Hemocyte markers hml-gal4/UAS-GFP (C, green) and Pxn (D, red),
crystal cell markers Lz (E; red) and Black cells (Bc, F),
and plasmatocyte marker P1 (G, red) are limited to the cortical zone. (H,I)
The uncharacterized GFP-trap line ZCL2826 (H, green) and the
transcription factor Cut (I, red) are preferentially expressed in the cortical
zone. (J,K) Lamellocytes, when present, are found in the cortical zone.
Lamellocytes can be distinguished by morphology in G147 background
(J,K, green, arrows) and by co-localization with msn-lacZ (K, red,
arrow). To-pro-3 (blue) marks nuclei. All lymph glands presented are of the
third larval instar.
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